SAB4200572

Anti-Neuron-Specific Enolase (NSE) antibody, Mouse monoclonal

clone NSE-P2, purified from hybridoma cell culture

• 同義詞: Monoclonal Anti-2-phospho-D-glycerate hydro-lyase, Monoclonal Anti-2-phospho-D-glycerate hydrolyase, Monoclonal Anti-Enolase 2 (gamma, neuronal), Monoclonal Anti-NSE, Monoclonal Anti-Neural enolase, Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse, Monoclonal Anti-gamma-enolase
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: NSE-P2, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~47 kDa
• 物種活性: human, rat
• 濃度: ~1 mg/mL
• 技術: immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.; western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts.
• 同型: IgG1
• UniProt登錄號: P09104
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ENO2(2026)

描述

一般說明

Monoclonal Anti-Neuron-specific Enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neuron-specific enolase (NSE) is present in the brain especially in the neuroendocrine cells and neurons. It is a cytosolic enzyme, that exists as a dimer and belongs to the family of enolase enzymes. NSE gene is mapped to human chromosome12p13.31.

特異性

Monoclonal Anti-Neuron-specific Enolase (NSE) recognizes human and rat NSE.

免疫原

synthetic peptide corresponding to an internal sequence of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

應用

Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse may be used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry

生化／生理作用

Neuron-specific enolase (NSE) catalyzes the production of phosphoenolpyruvate from 2-phospho-D-glycerate in the glycolytic pathway. NSE is a specific neurobiochemical marker of brain damage after traumatic brain injury, hypoxic encephalopathy and stroke. Moreover, NSE has been suggested to be a general circulating marker for neuroendocrine tumors. Interestingly, high levels of NSE were also reported in breast cancer upon exposure to the environmental pollutants arsenite and cadmium.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

儲存和穩定性

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable