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S6022

Sigma-Aldrich

葡聚糖凝胶 ® G-50

BioReagent, for molecular biology, DNA grade, medium

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.52

等級

DNA grade
for molecular biology

品質等級

產品線

BioReagent

形狀

beads

膨潤

1 g swells to 9-11 mL

珠直徑

50-150 μm

孔徑

exclusion limit (20 bp dsDNA; (rA)20)

pH值

2-10

異物活動

DNase, RNase, none detected

儲存溫度

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI 密鑰

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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一般說明

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

應用

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.

特點和優勢

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

法律資訊

Sephadex is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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R Symula et al.
Molecular phylogenetics and evolution, 47(2), 569-580 (2008-03-20)
Geographic patterns of species diversity in southeast Australia have been attributed to changes in Pleistocene climate, but related phylogeographic patterns and processes are relatively understudied. 12S and 16S mitochondrial DNA sequences in Crinia signifera populations were used to infer historical
Alisha K Holloway et al.
The American naturalist, 167(4), E88-101 (2006-05-04)
Polyploidization is one of the few mechanisms that can produce instantaneous speciation. Multiple origins of tetraploid lineages from the same two diploid progenitors are common, but here we report the first known instance of a single tetraploid species that originated
The history of a Nearctic colonization: molecular phylogenetics and biogeography of the Nearctic toads (Bufo)
Pauly GB, et al.
Evolution, 58(11), 2517-2535 (2004)
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the

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