推薦產品
異物活動
exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free
protease and sphingomyelinase, essentially free
品質等級
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.
應用
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides. It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.
生化／生理作用
Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids. It condenses fatty acids to sphingosine to generate ceramide. The enzyme mainly acts on neutral and acidic glycosphingolipids.
單位定義
One unit will hydrolyze 1 μmol of asialo GM1 per minute at pH 6.0 at 37 °C.
外觀
Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Characterization of the reversible nature of the reaction catalyzed by sphingolipid ceramide N-deacylase. A novel form of reverse hydrolysis reaction.
Kita K
European Journal of Biochemistry, 268(3), 592-602 (2001)
Sulfatide Analysis by Mass Spectrometry for Screening of Metachromatic Leukodystrophy in Dried Blood and Urine Samples.
Spacil Z
Clinical Chemistry, 62(1), 279-286 (2016)
A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms.
Ito M
The Journal of Biological Chemistry, 270(41), 24370-24374 (1995)
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