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重要文件

RAB0300

Sigma-Aldrich

小鼠 IL-4 ELISA 试剂盒

for cell and tissue lysates

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.32

物種活性

mouse

包裝

kit of 96 wells (12 strips x 8 wells)

技術

ELISA: suitable
capture ELISA: suitable

輸入

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.819-200 pg/mL

檢測方法

colorimetric

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

mouse ... Il4(16189)

一般說明

The Mouse IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-4 in cell lysate and tissue lysate.

免疫原

Recombinant Mouse IL-4

應用

For research use only. Not for use in diagnostic procedures.
Mouse IL-4 ELISA kit has been used for determining the levels of IL-4 in serum sample.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

生化/生理作用

IL-4 (interleukin 4) is a pleiotropic cytokine. It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior.

其他說明

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABLYSIS12X Cell Lysis Buffer (Item J)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

象形圖

Corrosion

訊號詞

Warning

危險聲明

防範說明

危險分類

Met. Corr. 1

儲存類別代碼

8B - Non-combustible corrosive hazardous materials


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Flavonoid Glycosides of Polygonum capitatum protect against inflammation associated with Helicobacter pylori infection.
Zhang Shu
PLoS ONE, 10(5) (2015)
Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4.
Hasbold J
European Journal of Immunology, 28, 1040-1051 (1998)
Nasal mast cells in perennial allergic rhinitics exhibit increased expression of the Fc epsilonRI, CD40L, IL-4, and IL-13, and can induce IgE synthesis in B cells.
Pawankar R
The Journal of Clinical Investigation, 99, 1492-1492 (1997)
IL-4 Knock Out Mice Display Anxiety-Like Behavior.
Moon ML
Behavior Genetics, 45, 451-460 (2015)
Ile50Val variant of IL4R alpha upregulates IgE synthesis and associates with atopic asthma.
Mitsuyasu H
Nature Genetics, 19, 119-120 (1998)

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