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Merck
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重要文件

R7131

Sigma-Aldrich

RPMI 1640氨基酸溶液(50×)

Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture

同義詞:

Amino Acid Supplement

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About This Item

分類程式碼代碼:
12352209
NACRES:
NA.75

品質等級

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

RPMI 1640氨基酸溶液(50×)是氨基酸浓缩液,用于补充以Roswell Park Memorial Institute培养基(RPMI)为基础配方的细胞培养基。

應用

RPMI 1640氨基酸溶液(50×)作为补充剂已用于:
  • 补充M9基础培养基,用于培养细菌菌株SX701和JE116
  • 体外培养哺乳母牛血中性粒细胞
  • 补充非氨基酸Roswell Park Memorial Institute培养基(RPMI),对HeLa细胞进行氨基酸刺激

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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D Kazyken et al.
The Journal of biological chemistry, 297(4), 101100-101100 (2021-08-22)
The mechanistic target of rapamycin (mTOR) complex 2 (mTORC2) signaling controls cell metabolism, promotes cell survival, and contributes to tumorigenesis, yet its upstream regulation remains poorly defined. Although considerable evidence supports the prevailing view that amino acids activate mTOR complex
Preeti Ashok et al.
Frontiers in bioengineering and biotechnology, 8, 571425-571425 (2020-11-17)
Functional heart cells and tissues sourced from human pluripotent stem cells (hPSCs) have great potential for substantially advancing treatments of cardiovascular maladies. Realization of this potential will require the development of cost-effective and tunable bioprocesses for manufacturing hPSC-based cell therapeutics.
Piero Dalle Pezze et al.
Nature communications, 7, 13254-13254 (2016-11-22)
Amino acids (aa) are not only building blocks for proteins, but also signalling molecules, with the mammalian target of rapamycin complex 1 (mTORC1) acting as a key mediator. However, little is known about whether aa, independently of mTORC1, activate other
Daniel Bloch Hansen et al.
The Journal of membrane biology, 245(2), 77-87 (2012-03-03)
The present work was initiated to investigate regulation of the taurine transporter TauT by reactive oxygen species (ROS) and the tonicity-responsive enhancer binding protein (TonEBP) in NIH3T3 mouse fibroblasts during acute and long-term (4 h) exposure to low-sodium/hypo-osmotic stress. Taurine

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