推薦產品
特異性
识别序列： 5′-G/AATTC-3′
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
應用
Eco RI 是一种限制性内切酶，在分子生物学应用中用于切割识别站点 5′ 的 DNA-G/AATTC-3′，产生带有 5′ 的片段-粘性终点。
其他說明
提供 10x 限制性内切酶缓冲液 SH (B3657)。
备注： 避免次优反应条件，如低盐、高 pH 值 ( 8.0) 和高甘油 ( 5%)，因为它们将改变 EcoRI 特异性并沉淀星号活性。
單位定義
一个单位表示在37°C下1小时内，在总体积为50 ml的1x限制酶消化缓冲液SH中完全切割1 mg λDNA的酶活性。1 mg pBR322 DNA可通过2个单位的EcoR I完全消化。
外觀
10 mM Tris-HCl 溶液，pH 值 7.2、1 mM EDTA、200 mM NaCl、0.5 mM 二硫赤藓糖醇、50% 甘油 (v/v)、0.2%Triton X-100 (v/v)，4°C
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential
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The generation of transgenic animals is an essential part of research in Caenorhabditis elegans. One technique for the generation of these animals is biolistic bombardment involving the use of DNA-coated microparticles. To facilitate the identification of transgenic animals within a
TALENs and ZFNs are associated with different mutation signatures.
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