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PROTRA

Millipore

ProteoPrep® 还原和烷基化试剂盒

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

儲存期限

≥1 yr (when stored at 2-8°C)

品質等級

200

儲存溫度

2-8°C

相關類別

應用

ProteoPrep Reduction & Alkylation Kit含有便于还原和烷基化二硫键所需的必要试剂，适于2D凝胶电泳样本制备。此类试剂兼容离液提取试剂，包装方便，可节省时间并提高效率。在该试剂盒中还原剂三丁基膦(TBP)和烷化剂碘乙酰胺各五管。

特點和優勢

  • 三丁基膦以200 mM N-甲基-2-吡咯烷酮溶液形式安全提供
  • 蛋白样本的还原和烷化有助于提高2D斑点的分辨率
  • 方便的成分包装，有助于节省时间、提高效率
  • 兼容离液提取试剂，简化了样本制备过程

法律資訊

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Shikha Shikha et al.
Microorganisms, 10(5) (2022-05-29)
Mitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising questions about its evolution

文章

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

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