P9318

Monoclonal Anti-Plectin antibody produced in mouse

clone 7A8, ascites fluid

• 同義詞: Anti-EBS1, Anti-EBS5A, Anti-EBS5B, Anti-EBS5C, Anti-EBS5D, Anti-EBSMD, Anti-EBSND, Anti-EBSO, Anti-EBSOG, Anti-EBSPA, Anti-HD1, Anti-LGMD2Q, Anti-LGMDR17, Anti-PCN, Anti-PLEC1, Anti-PLEC1b, Anti-PLTN
• MDL號碼: MFCD00164718
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 7A8, monoclonal
• 分子量: antigen 300 kDa
• 包含: 15 mM sodium azide
• 物種活性: marsupial (Ptoorous tridactylis PtK₂ cell line), rat
• 技術: dot blot: suitable; immunocytochemistry: suitable; immunohistochemistry (frozen sections): suitable; indirect ELISA: suitable; indirect immunofluorescence: 1:200 using unfixed frozen sections of rat heart; microarray: suitable; western blot: suitable
• 同型: IgG1
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: rat ... Plec1(64204)

描述

一般說明

Monoclonal Anti-Plectin (mouse IgG1 isotype) is derived from the 7A8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Plectin is an abundant, high molecular weight, cytomatrix protein (300 kDa) found in a wide variety of tissue and cell types. Plectin is found in stratified and non-stratified epithelia, fibroblasts, endothelial cells and astrocytes, as well as in striated, smooth and cardiac muscle, but not in neurons.

特異性

The antibody recognizes an epitope located in the middle section of the rod domain of the plectin molecule, approximately halfway between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as this antibody epitope. The antibody specifically labels the 300 kDa band of plectin, however, lower M.W. band(s) may also be detected. The antibody stains filamentous structures on immunohistochemical staining of frozen tissue sections. The antibody inhibits the interaction of plectin with vimentin and lamin B. An additional application for this antibody is immunoelectronmicroscropy.

免疫原

plectin from rat glioma C6 cells

應用

Monoclonal Anti-Plectin antibody produced in mouse has been used in:

• ELISA
• immunoblot
• dot blot
• immunocytochemistry
• immunoelectron microscopy

生化／生理作用

Plectin plays a role in the cross-linking of intermediate filaments, the interlinking of intermediate filaments with microtubules and microfilaments and the anchoring of intermediate filaments to the plasma membrane and the nuclear membrane. It is a part of the signal transduction mechanism involving kinases A and C, because in vitro as well as in vivo phosphorylation of the protein by those kinases differentially affected its binding affinities to vimentin and lamin B.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable