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Merck
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Key Documents

F0397

Sigma-Aldrich

Anti-Cytokeratin pan-FITC antibody, Mouse monoclonal

clone PCK-26, purified from hybridoma cell culture

同義詞:

Anti-CK16-Fluorescein isothiocyanate, Anti-FNEPPK, Anti-K16, Anti-K1CP, Anti-KRT16A, Anti-NEPPK, Anti-PC1

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

FITC conjugate

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

PCK-26, monoclonal

形狀

buffered aqueous solution

技術

direct immunofluorescence: 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues
immunohistochemistry (frozen sections): suitable

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.
FITC Monoclonal Anti-Pan Cytokeratin is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal anti-pan cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II, neutral-to-basic subfamily. Cytokeratin peptide 5 is the primary type II keratin in stratified epithelia while cytokeratin type 8 is a major type II keratin in simple epithelia. Cytokeratin 6 is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover.

特異性

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

免疫原

cytokeratin from human epidermis.

應用

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse has been used in:
  • immunohistochemistry
  • staining chicken intestinal cells
  • immunofluorescence staining

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse is suitable for direct immunofluorescence at a dilution of 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues and immunohistochemistry (frozen sections).

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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存取文件庫

Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro
Byrne CM, et al.
Microbiology, 561-569 (2007)
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Breast cancer cells frequently home to the bone, but the mechanisms controlling tumor colonization of the bone marrow remain unclear. We report significant enrichment of bone-disseminated estrogen receptor positive human MCF7 cells by 17 β-estradiol (E2) following intracardiac inoculation. Using flow
Rebecca A Brockman-Schneider et al.
Journal of immunological methods, 339(2), 264-269 (2008-10-01)
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Serial culture of murine primary airway epithelial cells and ex vivo replication of human rhinoviruses
Brockman-SRA, et al.
Journal of Immunological Methods, 339(2), 264-269 (2008)
Interleukin 22 prevents lipopolysaccharide-induced preterm labor in mice
Dambaeva S, et al.
Biology of Reproduction, 98(3), 299-308 (2018)

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