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Key Documents

P7059

Sigma-Aldrich

磷酸盐缓冲溶液

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

同義詞:

Phosphate buffered saline, PBS

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About This Item

MDL號碼:
分類程式碼代碼:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

磷酸盐缓冲溶液, 10× PBS for Western blots and IP

等級

for molecular biology

品質等級

無菌

0.2 μm filtered

形狀

liquid

技術

immunoprecipitation (IP): suitable
western blot: suitable

pH值

7.2-7.6(1x solution)

儲存溫度

room temp

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一般說明

磷酸盐缓冲盐水(PBS)通常用作蛋白质印迹和免疫沉淀(IP)程序中的洗涤缓冲液,以及用于一般分子生物学应用的细胞裂解物的样品制备。

應用

适用于蛋白质印迹和IP的洗涤缓冲液,以及细胞裂解液样品制备。

成分

10x PBS 含有:
  • 30mM 磷酸钠
  • 1.5M 氯化钠
  • 10.5mM 磷酸钾

準備報告

使用分子生物级水将 10x PBS 稀释至 1x工作溶液。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Imran Tarique et al.
Animals : an open access journal from MDPI, 9(11) (2019-11-07)
The ductuli efferentes (DE) form a transit passage for the passage of spermatozoa from the rete testis to the epididymis. After spermiation, various epithelial secretory proteins are transferred via extracellular vesicles (EVs) to the spermatozoa for their maturation and long-term
Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Caroline R Archer et al.
Scientific reports, 8(1), 10160-10160 (2018-07-07)
Pharmaceutical agents despite their efficacy to treat disease can cause additional unwanted cardiovascular side effects. Cardiotoxicity is characterized by changes in either the function and/or structure of the myocardium. Over recent years, functional cardiotoxicity has received much attention, however morphological

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