推薦產品
等級
for molecular biology
品質等級
形狀
lyophilized powder
技術
Southern blotting: suitable
異物活動
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
儲存溫度
2-8°C
一般說明
Denhardt溶液是一种阻断剂,通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液,可稀释至任何浓度,用于各种预杂交和杂交缓冲液。
應用
Denhardt溶液已被用于:
- 作为杂交缓冲液的组分,用于脑切片的杂交
- 用于原位杂交过氧化物酶-(抗过氧化物酶法-全标本包埋)(ISHPAP-WM)过程
- 用于杂交混合物的制备
适合用于Southern和Northern印迹膜的封闭缓冲液。
生化/生理作用
Denhardt溶液和明胶-铬明矾可用于处理染色体,从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。
成分
每管Denhardt粉末含有50mg牛血清白蛋白(BSA)、50mg Ficoll和50mg聚乙烯吡咯烷酮(PVP)。
準備報告
制备50倍浓缩液,将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。
相關產品
產品號碼
描述
訂價
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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