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Key Documents

P7032

Sigma-Aldrich

丙酮酸脱氢酶 来源于猪心脏

buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)

同義詞:

PDH, Pig heart pyruvate dehydrogenase

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

buffered aqueous glycerol solution

品質等級

比活性

0.1-1.0 units/mg protein (Lowry)

濃度

≥5.0 mg/mL

雜質

polyethylene glycol, trace

異物活動

α-ketoglutarate dehydrogenase ≤15%

運輸包裝

dry ice

儲存溫度

−20°C

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應用

丙酮酸脱氢酶有助于将糖酵解代谢途径与柠檬酸循环进行连接,并通过NADH释放能量。这种酶用于研究有氧训练如何影响丙酮酸脱氢酶,还用于研究丙酮酸脱氢酶激酶和丙酮酸脱氢酶复合物之间的相互作用,以及丙酮酸脱氢酶复合物的功能和调节。P7032产品以缓冲的甘油水溶液形式提供,来源于猪心脏。
该酶已用于初级酶测定以测试某些化合物还原PDK-(丙酮酸脱氢酶激酶)催化的PDC(丙酮酸脱氢酶复合物)失活的能力。

生化/生理作用

丙酮酸脱氢酶可参与到丙酮酸脱羧酶复合物(PDC)中丙酮酸的脱羧和硫辛酸的还原乙酰化。丙酮酸脱氢酶激酶(PDK)可使丙酮酸脱氢酶失活,而丙酮酸脱氢酶磷酸酶则能激活丙酮酸脱氢酶

單位定義

在30℃、pH 7.4、饱和水平的辅酶A存在的条件下,一单位每分钟可将1.0 μm摩尔的β-NAD转化成β-NADH。

外觀

以 50% 甘油溶液的形式提供,含有 ~9mg/mL 牛血清白蛋白、30% 蔗糖、1.5mM EDTA、1.5mM EGTA、1.5mM 2-巯基乙醇、0.3 TRITON® X-100、0.003% 叠氮化钠和 15mM 磷酸钾,pH 值为 6.8

危險聲明

防範說明

危險分類

Aquatic Chronic 3

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Insulin stimulation of pyruvate dehydrogenase in an isolated plasma membrane-mitochondrial mixture occurs by activation of pyruvate dehydrogenase phosphatase.
D A Popp et al.
The Journal of biological chemistry, 255(16), 7540-7543 (1980-08-25)
Function and regulation of mammalian pyruvate dehydrogenase complex. Acetylation, interlipoyl acetyl transfer, and migration of the pyruvate dehydrogenase component.
R L Cate et al.
The Journal of biological chemistry, 254(5), 1659-1665 (1979-03-10)
T D Aicher et al.
Bioorganic & medicinal chemistry letters, 9(15), 2223-2228 (1999-08-28)
Several oximes of triterpenes with a 17-beta hydroxyl and abietane derivatives are inhibitors of pyruvate dehydrogenase kinase (PDK) activity. The oxime 12 and dehydroabietyl amine 2 exhibit a blood glucose lowering effect in the diabetic ob/ob mouse after a single
Naoyuki Makita et al.
The Journal of pharmacy and pharmacology, 69(1), 43-51 (2016-10-21)
Recently, there has been a growing interest in the mechanism of action of dichloroacetate (DCA) for T-cell differentiation; however, this mechanism has not been elucidated in detail. Therefore, this study aimed to investigate the mechanism of action of DCA for
Suzanne D DeBrosse et al.
Molecular genetics and metabolism, 107(3), 394-402 (2012-10-02)
Pyruvate dehydrogenase complex (PDC) deficiency is a relatively common mitochondrial disorder that primarily presents with neurological manifestations and lactic acidemia. We analyzed the clinical outcomes and neurological features of 59 consented symptomatic subjects (27 M, 32 F), who were confirmed

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