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應用
丙酮酸脱氢酶有助于将糖酵解代谢途径与柠檬酸循环进行连接,并通过NADH释放能量。这种酶用于研究有氧训练如何影响丙酮酸脱氢酶,还用于研究丙酮酸脱氢酶激酶和丙酮酸脱氢酶复合物之间的相互作用,以及丙酮酸脱氢酶复合物的功能和调节。P7032产品以缓冲的甘油水溶液形式提供,来源于猪心脏。
该酶已用于初级酶测定以测试某些化合物还原PDK-(丙酮酸脱氢酶激酶)催化的PDC(丙酮酸脱氢酶复合物)失活的能力。
生化/生理作用
丙酮酸脱氢酶可参与到丙酮酸脱羧酶复合物(PDC)中丙酮酸的脱羧和硫辛酸的还原乙酰化。丙酮酸脱氢酶激酶(PDK)可使丙酮酸脱氢酶失活,而丙酮酸脱氢酶磷酸酶则能激活丙酮酸脱氢酶。
單位定義
在30℃、pH 7.4、饱和水平的辅酶A存在的条件下,一单位每分钟可将1.0 μm摩尔的β-NAD转化成β-NADH。
外觀
以 50% 甘油溶液的形式提供,含有 ~9mg/mL 牛血清白蛋白、30% 蔗糖、1.5mM EDTA、1.5mM EGTA、1.5mM 2-巯基乙醇、0.3 TRITON® X-100、0.003% 叠氮化钠和 15mM 磷酸钾,pH 值为 6.8
危險聲明
防範說明
危險分類
Aquatic Chronic 3
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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