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Key Documents

P5860

Sigma-Aldrich

蛋白酶 来源于芽孢杆菌

liquid, ≥8 U/g

同義詞:

Esperase® 8.0L

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

liquid

品質等級

比活性

≥8 U/g

分子量

20--30 kDa

儲存溫度

2-8°C

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相關類別

應用

蛋白酶是一种通过水解肽键而分解蛋白质的酶。产物P5860是从芽孢杆菌属(Bacillus sp.)中分泌的。它是一种丝氨酸型蛋白酶,在高温和高pH下具有出色的活性。蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热灭活动力学。 该酶已被作为一种环境友好的非热技术,用于测试紫外线辐射,以便对食品工业中的酶进行灭活。

生化/生理作用

蛋白酶通过水解肽键而分解蛋白质。丝氨酸活性位点抑制剂(如苯基甲基磺酰氟(PMSF)和氟磷酸二异丙酯)可使蛋白酶失活。从芽孢杆菌属(Bacillus sp.)分泌的蛋白酶的分子量通常为20,000至30,000。它们通常由Ca2+ 稳定,并具有较高的等电点

法律資訊

Novozyme 公司产品。
Esperase is a registered trademark of Novozymes Corp.

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


分析證明 (COA)

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D R Durham et al.
Journal of bacteriology, 169(6), 2762-2768 (1987-06-01)
An alkalophilic Bacillus sp., strain GX6638 (ATCC 53278), was isolated from soil and shown to produce a minimum of three alkaline proteases. The proteases were purified by ion-exchange chromatography and were distinguishable by their isoelectric point, molecular weight, and electrophoretic
Elodie Lefebvre et al.
Materials science & engineering. C, Materials for biological applications, 82, 210-216 (2017-10-14)
Chronic wound colonization by bacterial biofilms is common and can cause various complications. An anti-biofilm strategy was developed around the co-entrapment of a commercially available antiseptic, PHMB (polyhexamethylene biguanide 4mgmL
Vadim Volkov et al.
International journal of biological macromolecules, 85, 476-486 (2016-01-13)
The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by (31)P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation
Effect of UV light on microbial proteases: From enzyme inactivation to antioxidant mitigation.
Lante A, et al.
Innovative Food Science & Emerging Technologies, 17, 130-134 (2013)

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