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Key Documents

P3895

Sigma-Aldrich

磷酸酶(碱性) 来源于人类胎盘

≥10 units/mg solid (in glycine buffer)

同義詞:

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

solid

品質等級

比活性

≥10 units/mg solid (in glycine buffer)

UniProt登錄號

儲存溫度

−20°C

基因資訊

human ... ALPP(250)

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應用

碱性磷酸酶可与抗体及其他蛋白质偶联而用于ELISA、Western blotting和组织化学检测。它通常用于使蛋白质和核酸去磷酸化。当需要高灵敏度时,可用于蛋白标记。碱性磷酸酶也可用于使DNA或RNA的5′-末端去磷酸化以防止自连。在用碱性磷酸酶去磷酸化后,DNA或RNA也可用放射性标记的磷酸盐(通过T4多核苷酸激酶)进行标记。

生化/生理作用

碱性磷酸酶可使蛋白质和核酸去磷酸化。人胎盘碱性磷酸酶(PLAP)具有独特的残基Glu-429。它是一种可被L-Phe、L-Trp和L-Leu无竞争性抑制的变构酶。PLAP是经过翻译后修饰的。磷脂酰肌醇特异性磷脂酶C可能参与到了PLAP的释放。PLAP可能在细胞分裂中发挥作用,并参与搭配母体IgG向胎儿的转移

單位定義

在pH 10.4,37 °C条件下,一个单位可在每分钟水解1 μ摩尔的4-硝基苯基磷酸酯。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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M H Le Du et al.
The Journal of biological chemistry, 276(12), 9158-9165 (2001-01-01)
Human placental alkaline phosphatase (PLAP) is one of three tissue-specific human APs extensively studied because of its ectopic expression in tumors. The crystal structure, determined at 1.8-A resolution, reveals that during evolution, only the overall features of the enzyme have
Ashleigh M Philp et al.
Scientific reports, 7(1), 4042-4042 (2017-06-24)
The purpose of this study was to determine the effect of adiposity on the architecture and composition of hip OA subchondral bone, and to examine the pathological role of adipokines. Femoral heads were collected from normal-weight or over-weight/obese patients with
Jong Kil Lee et al.
The Journal of experimental medicine, 211(8), 1551-1570 (2014-07-23)
In Alzheimer's disease (AD), abnormal sphingolipid metabolism has been reported, although the pathogenic consequences of these changes have not been fully characterized. We show that acid sphingomyelinase (ASM) is increased in fibroblasts, brain, and/or plasma from patients with AD and
A D Bakker et al.
Journal of dental research, 93(4), 394-399 (2014-02-05)
Mechanosensitive osteocytes regulate bone mass in adults. Interleukin 6 (IL-6), such as present during orthodontic tooth movement, also strongly affects bone mass, but little is known about the effect of IL-6 on osteocyte function. Therefore we aimed to determine in
M D McKee et al.
Journal of dental research, 92(8), 721-727 (2013-05-23)
Phosphatases are involved in bone and tooth mineralization, but their mechanisms of action are not completely understood. Tissue-nonspecific alkaline phosphatase (TNAP, ALPL) regulates inhibitory extracellular pyrophosphate through its pyrophosphatase activity to control mineral propagation in the matrix; mice without TNAP

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