P3326

Platelet-Derived Growth Factor-AB 人

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 同義詞: PDGF-AB
• MDL號碼: MFCD00213024
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 產品名稱: Platelet-Derived Growth Factor-AB 人, PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 化驗: ≥97% (SDS-PAGE and N-terminal analysis)
• 形狀: lyophilized powder
• 效力: 10-100 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: 27-31 kDa
• 包裝: pkg of 10 μg; pkg of 50 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: ≤1 EU/μg
• UniProt登錄號: P04085, P01127
• 儲存溫度: −20°C
• 基因資訊: human ... PDGFA(5154) , PDGFB(5155)

描述

一般說明

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.

應用

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

生化／生理作用

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.

其他說明

Heterodimer of the A and B chains of PDGF disulfide linked.

外觀

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

分析報告

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)