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P2983

Millipore

高灵敏度抗-FLAG® M2抗体包被 96 孔板

96-well, clear, polystyrene, flat bottom plate

同義詞:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.32

抗體產品種類

primary antibodies

品質等級

200

材料

polystyrene

無性繁殖

monoclonal

儲存期限

Unopened plates are stable for 2 years. Once opened they are stable for 2 weeks.

技術

ELISA: suitable

同型

IgG1

容量

100-300 ng/well

儲存溫度

2-8°C

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相關類別

一般說明

抗-FLAG® HS，M2涂层板的设计可确保比其他孔板更高的结合能力。这是由于板上含有抗-FLAG® M2小鼠单克隆IgG1抗体。抗体经由抗体的Fc部分共价连接至微量滴定板的表面。

應用

ANTI-FLAG®高灵敏、M2包被96孔板可用于酶联免疫吸附测定。它是捕获和检测FLAG®标签融合蛋白的即用型便捷平台。ANTI-FLAG® M2抗体通过Fc部分共价连接孔板表面，可检测1 ng FLAG融合蛋白/孔，最多300ng/孔。适用于表达筛选、蛋白质互作研究和ELISA分析。ANTI-FLAG®P2983高灵敏M2包被多孔板在Sigma′ GMP设施中按ISO 9002标准生产，采用聚苯乙烯平底板。

更多产品信息，请访问我们的FLAG®应用页面。

儲存和穩定性

微孔板打开后，应当在2- 8 °C下以干燥剂储存，并在两星期内使用。

其他說明

组分：
提供的微孔板为96孔板，底部和侧面是透明的。
包被：
抗FLAG® M2小鼠单克隆抗体IgG1在200 ml/孔的反应体积下包被。
封闭：
为方便使用，微孔是以含有牛血清蛋白的复合物溶液按275 至300 ml/孔的量预先封闭。
特异性：
微孔板对FLAG表位有特异性，无论其在融合蛋白中的位置如何：氨基末端、蛋氨酸氨基末端、羧基末端或内部。与表位的结合不依赖于Ca2+。
灵敏度：
以磷酸对硝基苯酯（pNPP）为底物在ELISA中可以检测出1 ng/孔的对照融合蛋白。
容量：
研究表明可以捕获100至300ng/孔的FLAG融合蛋白。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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分析證明 (COA)

Lot/Batch Number
SLBS2649V
SLBM0677V
SLBH4623V
SLBD3336V
121M6000

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Lilian A Patrón et al.
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We genetically characterized the synaptic role of the Drosophila homologue of human DCAF12, a putative cofactor of Cullin4 (Cul4) ubiquitin ligase complexes. Deletion of Drosophila DCAF12 impairs larval locomotion and arrests development. At larval neuromuscular junctions (NMJs), DCAF12 is expressed
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Owing to the spread of multidrug resistance (MDR) and extensive drug resistance (XDR), there is a pressing need to identify potential targets for the development of more-effective anti-M. tuberculosis (Mtb) drugs. PafA, as the sole Prokaryotic Ubiquitin-like Protein ligase in
Mihaly Mezei et al.
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To gain further insight into the binding of the normal and variant human TSHβ subunits (TSHβ and TSHβv), we modeled the 2 monomeric proteins and studied their interaction with the TSH receptor ectodomain (TSHR-ECD) using molecular dynamics simulation Furthermore, analyzed
Natalie F Shanks et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(36), 12104-12120 (2014-09-05)
Cornichon homologs (CNIHs) are AMPA-type glutamate receptor (AMPAR) auxiliary subunits that modulate AMPAR ion channel function and trafficking. Mechanisms underlying this interaction and functional modulation of the receptor complex are currently unclear. Here, using proteins expressed from mouse and rat
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Shanks NF, Cais O, Maruo T, et al.
The Journal of Neuroscience, 34(36), 12104-12120 (2014)
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