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![鸟苷5′-[β,γ-亚氨基]三磷酸 三钠盐 水合物 ≥85% (HPLC), powder](/deepweb/assets/sigmaaldrich/product/structures/204/494/05808804-1ca7-44bf-b6c5-d4934dc7cb85/640/05808804-1ca7-44bf-b6c5-d4934dc7cb85.png)
生物源
bovine (calf) intestine
形狀
ammonium sulfate suspension
比活性
250-1000 units/mL packed gel
基質
beaded agarose
儲存溫度
2-8°C
一般說明
来自小牛肠的碱性磷酸酶是一种糖蛋白，分子量为140,000。
應用
小牛肠道碱性磷酸酶（CIAP）已用于通过分光光度法评估丹磺酰基-L-苯丙氨酸的抑制作用。 CIAP还被证明能够清除脂多糖（LPS）介导的炎症反应，这在治疗LPS介导的疾病中可能具有重要作用。
来自小牛肠的碱性磷酸酶CIP可去除DNA的5′末端的磷酸基团，有助于克隆技术，防止DNA自连接。它可以被高精氨酸抑制。
生化／生理作用
Sigma不溶性酶是通过使用常规“可溶”酶与惰性基质反应，获得保留原始酶活性的不溶性化合物而生成的。应用几乎不受限制。通过仔细选择“惰性”基质，可产生高活性的“树脂”或“凝胶”，它们与底物在合适介质中的瞬时接触，能够迅速催化酶反应。反应可以在间歇桨液反应器中进行，甚至可以在小柱中进行。无论是哪种情况，不溶性酶都不会污染反应混合物，可以很容易地将其从间歇桨液中滤出或留在色谱柱中。许多不稳定酶在不溶形式下可能会更加稳定。
包裝
包装规格取决于 DEA单位
單位定義
一个酶活性单位是指在pH 9.8、37°C下，每分钟水解1.0 mmole 对硝基苯磷酸盐所需的酶量。
外觀
悬浮于 2.0 M (NH4)2SO4，1 mM MgCl2，0.1 mM ZnCl2，pH 7
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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