P0072

Anti-phospho-PDCD4 (pSer⁶⁷) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-Neoplastic transformation inhibitor, Anti-Nuclear antigen H731, Anti-Programmed cell death 4, Anti-Protein 197/15a
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~51 kDa
• 物種活性: rat (predicted), mouse (predicted), human
• 濃度: ~1 mg/mL
• 技術: immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells starved for 48 hours and then treated with 20% FCS; western blot: 1-2 μg/mL using lystes of HEK-293T cells starved for 48 hours and then treated with 20% FCS
• UniProt登錄號: Q53EL6
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pSer67)
• 基因資訊: human ... PDCD4(27250) , SELP(6403) ; mouse ... Pdcd4(18569)

描述

一般說明

Programmed cell death 4 (PDCD4) also known as nuclear antigen H731-like, Protein 197/15a, neoplastic transformation inhibitor protein), is a tumor suppressor protein that is lost in progressed carcinomas of lung, breast, colon and prostate.

應用

Anti-phospho-PDCD4 (pSer67) antibody produced in rabbit has been used in immunoblotting.

生化／生理作用

Programmed cell death 4 (PDCD4) suppresses translation initiation by specifically inhibiting the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex and by competing with eIF4G, a second component of the translation initiation complex, for binding to eIF4A. In response to mitogens, PDCD4 was rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF-β (TRCP). It has been shown that the protein is specifically phosphorylated on Ser67 and Ser457 by Akt, both in vitro and in vivo. This phosphorylation causes nuclear translocation of PDCD4.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)