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重要文件

P0051

Sigma-Aldrich

Anti-PINK1 (N-terminal region) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

同義詞:

Anti-BRPK, Anti-PARK6, Anti-PTEN induced putative kinase 1

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen ~60 kDa

物種活性

human

包裝

antibody small pack of 25 μL

加強驗證

recombinant expression
Learn more about Antibody Enhanced Validation

濃度

~1.5 mg/mL

技術

western blot: 1-2 μg/mL using HEK-293T cell lysate expressing human PINK1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... PINK1(65018)

相關類別

一般說明

PTEN induced putative kinase 1 (PINK1) is a serine/threonine mitochondrial kinase. The 581 amino acid protein has an amino terminal mitochondrial target sequence, a putative transmembrane domain, a kinase domain and an autophosphorylation-regulating carboxy terminal domain. The gene encoding it is localized on human chromosome 1p36.12.

應用

Anti-PINK1 (N-terminal region) antibody produced in rabbit has been used in western blotting.

生化/生理作用

PINK1 (PTEN induced putative kinase 1) has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. Genetic studies in Drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 elicits protection in mouse primary neurons from the dopaminergic neurotoxin 1-methyl-4-phenylpyridine (MPP+)/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) both in vitro and in vivo. In response to enhanced proteasomal stress in vitro, PINK1 has been shown to be cleaved and localized to the mitochondria, and this correlates with increased expression of the processed PINK1 protein in Parkinson′s disease(PD) brain.
PTEN induced putative kinase 1 (PINK1) has a role in removing damaged mitochondria from cells. Mitochondrial damage triggers the accumulation of the protein in the outer mitochondrial membrane, wherein it undergoes autophosphorylation and dimerizes into a supermolecular protein complex. PINK1 then phosphorylates ubiquitin and tags damaged mitochondria for mitophagy. It negatively modulates glioblastoma growth. Mutations in the PINK1 gene have been associated with recessive, early-onset Parkinson′s disease. The protein is expressed in cancerous cells and has a role in cell survival.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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存取文件庫

Heterozygous PINK1 p.G411S increases risk of Parkinson?s disease via a dominant-negative mechanism
Brain (2017)
Constitutive Activation of PINK1 Protein Leads to Proteasome-mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy.
Akabane S
The Journal of Biological Chemistry (2016)
Update on Genetics of Parkinsonism
Shinsuke Fujioka Zbigniew K. Wszolek
Neurogenetics (2012)
PINK1 Is a Negative Regulator of Growth and the Warburg Effect in Glioblastoma.
Agnihotri S
Cancer Research (2016)
Acute focal brain damage alters mitochondrial dynamics and autophagy in axotomized neurons
Cavallucci V, et al.
Cell Death & Disease, 5(11), e1545-e1545 (2014)

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