MSP03

Membrane Scaffold Protein 1D1(-)

recombinant, expressed in E. coli, buffered aqueous solution

• 同義詞: Membrane Scaffold Protein
• 分類程式碼代碼: 12352200
• NACRES: NA.26

屬性

• 重組細胞: expressed in E. coli
• 品質等級: 200
• 化驗: ≥90% (SDS-GE)
• 形狀: buffered aqueous solution
• 分子量: 22,043.9 Da
• 溶解度: water: soluble
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Research area: Cell structure. Membrane Scaffold Protein 1D1 (MSP1D1) is a variant of MSP1 where the first eleven amino acids in the helix 1 portion are deleted of the original MSP1 sequence. It consists of an N-terminal histidine-tagged protein with a tobacco etch virus (TEV) protease cleavage site that is present between the protein sequence and histidine-tag. MSP1D1(–)variant of MSP1D1 lacks the histidine-tag and the TEV protease-recognition sequence.

應用

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

生化／生理作用

Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable. The concept of this technology is derived from high-densitylipoprotein (HDL) particles and apolipoprotein, their primary proteincomponent. The nano-disc is a non-covalent structure consisting of MSP that isgenetically engineered to mimic the activity of apolipoprotein A1 (Apo-A1) andphospholipid bilayer. In the presence of synthetic phospholipids, MSP1D1 canself-assemble into discoidal nanoparticlesknown as nano-discs. These nano-discs are suitable for studying membraneproteins and are applicable in the field of medicine and biotechnology.

法律資訊

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable