MSP05

Membrane Scaffold Protein 1E1D1 D73C

recombinant, expressed in E. coli, Cysteine substituted at position 73

• 分類程式碼代碼: 12352200
• NACRES: NA.26

屬性

• 重組細胞: expressed in E. coli
• 化驗: ≥90% (SDS-GE)
• 形狀: buffered aqueous solution
• 運輸包裝: ambient
• 儲存溫度: −20°C

描述

一般說明

Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).

The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E1D1 D73C variant of MSP1 differs from MSP1 in the following facets:

• It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence (which is known separately as MSP1D1).
• It repeats the Helix 4 portion (H4) of the original MSP1 sequence between the parent Helix 4 (H4) and Helix 5 (H5) segments of MSP1D1.
• It replaces one aspartic acid residue (D) with a cysteine (C) at position 73 of the original native A-1 sequence, located within the Helix 2 segment (H2).

應用

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

法律資訊

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable