MBD0052

Hemin solution

Ready-made microbial growth medium supplement, 0.5 g/L

• 同義詞: Chlorohemin solution, ChloroprotoporphyrinIX iron(III) solution, Ferriprotoporphyrin IX chloride Solution
• 分類程式碼代碼: 41106212
• NACRES: NA.21

屬性

• 品質等級: 200
• 形狀: liquid
• 用途: supplement for growth media
• 濃度: 0.5 g/L
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C

描述

一般說明

Hemin is a pro-oxidant, hemolysis derived-product. It is essential for the growth of heme-deficient organisms such as trypanosomatids. Hemin readymade solution is used as a culture-medium supplement for fastidious anaerobic microorganisms, shown to enhance the growth of many anaerobes including Bacteroides species and certain gram-positive non-spore forming organisms. It is present as a key ingredient compound in common anaerobic growth media, such as Brucella broth, Thioglycolate broth and Reinforced Clostridial Medium.

應用

Hemin solution may be used as a supplement in brain heart infusion-yeast extract-hemin (BHI-YH) medium to determine cholesterol-to-coprostanol reducing activity.

特點和優勢

• Convenient readymade solution to speed up media preparation
• Sterile solution
• Optimal mixture for growth of fastidious bacteria as well as diversified culturing of microbial samples

其他說明

1. For 100 ml of medium preparation, it is recommended to add 1 mL hemin ready-made solution after autoclaving the medium, to achieve a final concentration of 5 microgram/ml. The optimal concentration of the supplement may vary for each strain.
2. The solution is classified as light sensitive. 2. The solution should not be heated and must be added aseptically to autoclaved media cooled to 50-55°C. 3.
3. Select strains which show turbid growth (24-48 hr.) with Hemin supplementation: Porphyromonas gingivalis, Bifidobacterium longum, Clostridium perfringens, Bacteroides vulgatus and Fusobacterium nucleatum.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2