MAK536

Cytotoxicity Assay Kit

sufficient for 100 assays (bioluminescent)

• 分類程式碼代碼: 12161503
• NACRES: NA.84

屬性

• 品質等級: 100
• 應用: pharmaceutical
• 檢測方法: bioluminescent
• 相關疾病: cancer
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

Adenosine 5′-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.

特點和優勢

• As low as 50 cells can be quantified.
• Compatible with high-throughput handling systems.
• No wash and reagent transfer steps are involved.

適合性

The kit is suitable for:

• Cell proliferation: effects of cytokines, growth factor, nutrients.
• Cytotoxicity and apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.
• Drug discovery: high-throughput screening for anticancer drugs.

原則

The Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability, and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317,H412
• 防範說明: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• 危險分類: Aquatic Chronic 3 - Skin Sens. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3