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Merck
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重要文件

MAK515

Sigma-Aldrich

Oxaloacetate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

同義詞:

Oxaloacetate Quantification Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

檢測方法

colorimetric
fluorometric

相關疾病

cancer; neurological disorders

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Oxaloacetate (OAA) is an intermediate in the citric acid cycle and participates in gluconeogenesis. OAA is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate.

特點和優勢

  • Linear detection range: 7 to 400 µM for the colorimetric assay and 1 to 40 µM for the fluorometric assay.

適合性

The kit is suitable for oxaloacetate determination in plasma, serum, tissue and culture media.

原則

The Oxaloacetate Assay Kit provides a simple, direct and automation-ready procedure for measuring oxaloacetate concentration. OAA is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the oxaloacetate concentration in the sample.

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


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