跳轉至內容
Merck
全部照片(1)

文件

MAK471

Sigma-Aldrich

Urea Assay Kit III

sufficient for 100 colorimetric tests

同義詞:

Urea Quantification Kit

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

應用

food and beverages

檢測方法

colorimetric

相關疾病

diabetes; renal disease; gastrointestinal diseases; endocrinological disorders; genitourinary disorders; cardiovascular diseases; orthopedic diseases

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Urea is the major end product of protein catabolism in animals. Urea is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is useful in assessing kidney function. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g., congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency but may also result from over vigorous parenteral fluid therapy.

特點和優勢

  • The linear detection range of the kit is 50 to 1000 µM urea in the 96-well plate assay
  • Compatible with high-throughput handling systems.
  • Simple procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37 °C heater is needed.

適合性

The kit is suitable for detection of urea in biological samples such as plasma, serum, urine, bronchoalveolar lavage (BAL), and food/beverage samples such as milk.

原則

The Urea Assay Kit III is designed to directly measure urea in biological samples. In this assay, urease converts urea to ammonia and carbon dioxide. NADH is then converted to NAD+ in the presence of ammonia, α-ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm is directly proportional to the urea concentration in the sample.

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務