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Key Documents

MAK311

Sigma-Aldrich

过氧化物检测试剂盒

sufficient for 250 colorimetric tests

同義詞:

Hydrogen Peroxide Test Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

檢測方法

colorimetric

相關疾病

cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders

儲存溫度

2-8°C

一般說明

过氧化物(如过氧化氢H2O2)是分子氧代谢产生的活性氧类,可作为细胞内信使和氧化应激来源。细胞可通过多种机制产生过氧化氢,如嗜中性粒细胞和巨噬细胞(呼吸爆发)中由NOX介导的ROS形成过程,或在线粒体呼吸的电子泄漏过程中由超氧阴离子歧化酶生成。 异常生成的过氧化氢会引起细胞氧化损伤和一些疾病进程,如哮喘、动脉粥样硬化、骨质疏松和神经退行性病变。

應用

过氧化物检测试剂盒用于测量大鼠组织匀浆中的过氧化氢水平。

特點和優勢

兼容高通量操作系统。

適合性

适用于多种生物样品(如血清、柠檬酸盐-血浆、尿液、细胞裂解液和培养基)中无需预处理的过氧化物浓度的定量测定。

原則

过氧化物检测试剂盒提供简单且高通量的适应性测定法,用于定量测定无需预处理的多种生物样品(如血清、柠檬酸盐-血浆、尿液、细胞裂解液和培养基)中的过氧化物浓度。该检测利用Fe3+ −二甲酚橙显色反应,样品中的过氧化物将Fe2+氧化为Fe3+,(与二甲酚橙)形成可通过比色法(585 nm)测定的紫色复合物,与过氧化物浓度成正比。优化后的配方可减少原始样品中其他物质的干扰。1 μM H2O2的浓度相当于34 ng/mL或34 ppb。该试剂盒的检测范围为0.2–30 μM (7–1,020 ng/mL)H2O2(96孔板形式)。

象形圖

Corrosion

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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