MAK506

Peroxidase Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• 同義詞: Peroxidase Enzyme Activity Kit
• 分類程式碼代碼: 12352204
• NACRES: NA.84

屬性

• 品質等級: 100
• 檢測方法: colorimetric; fluorometric
• 相關疾病: cancer; cardiovascular diseases; neurological disorders
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

Peroxidase catalyze the following oxidationreduction reactions: ROOR′ + electron donor (2 e-) + 2H+ → ROH + R′OH For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi, and bacteria (cytochrome-c peroxidase like enzymes such as horseradish peroxidase).

特點和優勢

• Linear detection range: 2 to 50 U/L for the colorimetric assay and 0.1 to 5 U/L for the fluorometric assay.
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for peroxidase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

原則

The Peroxidase Assay Kit uses H2O2 and an electron donor dye that forms resorufin during the peroxidase reaction. The optical density at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is a direct measure of the enzyme activity.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H319
• 防範說明: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危險分類: Eye Irrit. 2
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1