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Key Documents

MAK145

Sigma-Aldrich

细胞内ROS检测试剂盒(红色荧光)

sufficient for 200 fluorometric tests (red)

同義詞:

Intracellular ROS Fluorescence Assay

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.25

用途

sufficient for 200 fluorometric tests (red)

檢測方法

fluorometric

相關疾病

neurological disorders; cancer

儲存溫度

−20°C

一般說明

活性氧 (ROS) 是由于有氧呼吸过程中氧气减少和细胞内各种酶系统产生的。在生理水平,活性氧有助于细胞信号和宿主防御。ROS 生成增加,高于生物系统的解毒能力,导致氧化应激和细胞损伤。对细胞的主要损伤是由 ROS 诱导的膜脂、必需蛋白质和 DNA 中的多不饱和脂肪酸等大分子的改变所致。ROS 与阿尔茨海默病、帕金森病、癌症、衰老等疾病状态有关。

應用

荧光细胞内活性氧 (ROS) 试剂盒已用于检测活细胞中的细胞内 ROS。

適合性

本试剂盒适于通过酶标仪或荧光显微镜检测细胞内的ROS。

原則

试剂盒提供灵敏的一步荧光测定方法,不用孵育1小时即可检测活细胞的胞内 ROS(特别是超氧化物和羟基自由基)。ROS与细胞透过性传感器反应,生成的荧光产物(λex = 520/λem = 605 nm)与 ROS 量成比例。这一测定可用荧光微板读取仪,在96孔或384孔多孔板中进行。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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