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MAK054

Sigma-Aldrich

α-酮戊二酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

檢測方法

colorimetric
fluorometric

相關疾病

cancer

儲存溫度

−20°C

一般說明

α-酮戊二酸（α-KG）是三羧酸（TCA）循环中的关键中间体，是由异柠檬酸脱氢酶对异柠檬酸的氧化脱脂作用形成的。 通过谷氨酸脱氢酶催化谷氨酸合成 α-KG 是TCA 循环的厌氧反应的关键步骤。α-KG 是重要的氮转运蛋白。这种 α-酮戊二酸测定试剂盒提供了一种简单、灵敏、快速的方法，用于量化各种样品中的 α-KG。

應用

α-酮戊二酸测定试剂盒已用于测定 α-酮戊二酸的活性。

適合性

适用于测定细胞和组织裂解物等多种生物样本中的a-酮戊二酸

原則

通过偶联酶测定法测定 α-KG浓度，其产生与存在的 α-KG成比例的比色（570nm）/荧光（λex = 535/λem = 587 nm）产物。该试剂盒的典型检测灵敏度为 0.01 至 10 nmoles。

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象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317,H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點（°F）

188.6 °F - closed cup

閃點（°C）

87 °C - closed cup

分析證明 (COA)

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Effect of NBC e1 deletion on renal citrate and 2-oxoglutarate handling.
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Isocitrate dehydrogenase 1 (IDH1) mutations are common in gliomas, acute myeloid leukemia, and chondrosarcoma. The mutation 'hotspot' is a single arginine residue, R132. The R132H mutant of IDH1 produces the 2-hydroxyglutarate (2-HG) carcinogen from α-ketoglutarate (α-KG). The reduction of α-KG
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