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MAK037

Sigma-Aldrich

NAD/NADH 定量试剂盒

sufficient for 100 colorimetric tests

同義詞:

NAD/NADH检测试剂盒

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

用途

sufficient for 100 colorimetric tests

檢測方法

colorimetric

相關疾病

cancer

儲存溫度

−20°C

一般說明

烟酰胺腺嘌呤二核苷酸(NAD)是一种参与多种氧化还原反应的酶辅因子。NAD作为电子载体,在氧化(NAD)和还原(NADH)形式之间循环。NAD除了在氧化还原反应中发挥作用外,在ADP(二磷酸腺苷)-核糖基化反应和作为去乙酰化酶的底物中也发挥着关键作用。

應用

NAD/NADH定量试剂盒已可用于,当存在曲美他嗪时,SIRT1(去乙酰化酶1)活性(与缺血再灌注损伤相关)对NAD+/NADH定量的影响。

適合性

适用于动物组织(肝、肾等)、细胞培养(贴壁细胞或悬浮细胞)、血清或尿液中NADH和NAD的检测。

原則

NAD/NADH定量试剂盒为敏感检测细胞内核苷酸、NAD(烟酰胺腺嘌呤二核苷酸)和NADH及它们的比值提供了一种方便的工具,无需从样品中纯化NAD/NADH。该试剂盒针对NAD和NADH,不检测NADP或NADPH。NADt(NAD和NADH)或NADH在比色法(450 nm)中定量。

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危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids


分析證明 (COA)

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