MAK479

NADP/NADPH Assay Kit

sufficient for 100 colorimetric tests

• 分類程式碼代碼: 12161503
• NACRES: NA.84

屬性

• 品質等級: 100
• 檢測方法: colorimetric
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

特點和優勢

• The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
• Compatible with high-throughput handling systems.
• Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.

適合性

The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.

原則

The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.

安全資訊

• 象形圖: GHS02,GHS05,GHS07
• 訊號詞: Danger
• 危險聲明: H225,H290,H315,H319,H336
• 防範說明: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Respiratory system
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 2