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Merck
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重要文件

MAK020

Sigma-Aldrich

果糖-6-磷酸检测试剂盒

sufficient for 100 fluorometric tests

同義詞:

Fructose-6-P Quantification Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 fluorometric tests

檢測方法

fluorometric

相關疾病

cancer

儲存溫度

−20°C

一般說明

果糖-6-磷酸(F6P)是一种通过磷酸葡萄糖异构酶异构化葡萄糖-6-磷酸产生的糖酵解途径中间体。F6P可被进一步磷酸化为果糖1,6-二磷酸,随后可裂解为磷酸甘油醛和磷酸二羟丙酮。F6P也可以从糖酵解分流至磷酸戊糖途径的非氧化分支,以生成用于核苷酸合成的戊糖磷酸。F6P水平在快速增殖的细胞(如癌细胞)中升高。果糖-6-磷酸测定试剂盒是一种高灵敏度和简单的基于荧光的方法,可用于对多种样品中的F6P进行定量。

應用

果糖-6-磷酸测定试剂盒已被用于测定果糖-6-磷酸(F-6-P) 和葡萄糖-6-磷酸(G6P)。

適合性

适用于对多种样品中的果糖-6-磷酸进行定量

原則

果糖-6-磷酸的浓度可通过偶联酶反应进行测定,该反应可产生荧光测定产物(λex = 535nm /λem = 587nm),其与存在的果糖-6-磷酸成比例。该试剂盒的一般灵敏度为0.01至0.5纳摩尔F6P。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點(°F)

188.6 °F - closed cup

閃點(°C)

87 °C - closed cup


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