推薦產品
用途
sufficient for 100 fluorometric tests
檢測方法
fluorometric
相關疾病
cancer
儲存溫度
−20°C
一般說明
果糖-6-磷酸(F6P)是一种通过磷酸葡萄糖异构酶异构化葡萄糖-6-磷酸产生的糖酵解途径中间体。F6P可被进一步磷酸化为果糖1,6-二磷酸,随后可裂解为磷酸甘油醛和磷酸二羟丙酮。F6P也可以从糖酵解分流至磷酸戊糖途径的非氧化分支,以生成用于核苷酸合成的戊糖磷酸。F6P水平在快速增殖的细胞(如癌细胞)中升高。果糖-6-磷酸测定试剂盒是一种高灵敏度和简单的基于荧光的方法,可用于对多种样品中的F6P进行定量。
應用
果糖-6-磷酸测定试剂盒已被用于测定果糖-6-磷酸(F-6-P) 和葡萄糖-6-磷酸(G6P)。
適合性
适用于对多种样品中的果糖-6-磷酸进行定量
原則
果糖-6-磷酸的浓度可通过偶联酶反应进行测定,该反应可产生荧光测定产物(λex = 535nm /λem = 587nm),其与存在的果糖-6-磷酸成比例。该试剂盒的一般灵敏度为0.01至0.5纳摩尔F6P。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點(°F)
188.6 °F - closed cup
閃點(°C)
87 °C - closed cup
從最近期的版本中選擇一個:
分析證明 (COA)
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文章
We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.
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