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Merck
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重要文件

M7633

Sigma-Aldrich

4-甲基伞形酮-α-D-半乳糖苷

≥98% (TLC)

同義詞:

MU-alpha-GAL

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About This Item

經驗公式(希爾表示法):
C16H18O8
CAS號碼:
分子量::
338.31
Beilstein:
94674
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.32

化驗

≥98% (TLC)

形狀

powder

溶解度

water: 50 mg/mL, clear, colorless to very faintly yellow

螢光

λex 317 nm; λem 374 nm (pH 9.1)
λex 360 nm; λem 449 nm (Reaction product)

儲存溫度

−20°C

SMILES 字串

CC1=CC(=O)Oc2cc(O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1

InChI 密鑰

YUDPTGPSBJVHCN-CHUNWDLHSA-N

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應用

4-甲基伞形基-α-D-吡喃半乳糖苷已用作人工底物,测定 α-半乳糖苷酶-A 活性。

生化/生理作用

4-甲基伞形基α-D-吡喃半乳糖苷是 α-半乳糖苷酶-A 的荧光底物。这种酶将底物转化为蓝色荧光产物甲基伞形花。通过分光光度法测定荧光强度。

準備報告

4-甲基伞形酮α-D-吡喃半乳糖苷可溶于水(50 mg/ml),形成无色至淡黄色的澄清溶液(加热处理后)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays
S J Park et al.
Applied and environmental microbiology, 61(5), 2027-2029 (1995-05-01)
With a spectrofluorometer, the length of the incubation time required in the fluorogenic assay was reduced to 12 h. The threshold emissions for reading the fluorogenic reaction by the spectrofluorometer were 5 and 10 U for lauryl tryptose broth media
S Raghavan et al.
Journal of inherited metabolic disease, 28(6), 1005-1009 (2006-01-26)
Globoid cell leukodystrophy is an inherited metabolic disorder of the central nervous system caused by deficiency of the lysosomal enzyme galactocerebrosidase. Haematopoietic stem cell transplantation is the only available effective treatment. The engraftment from normal donors provides competent cells able
Y Oda et al.
Analytical biochemistry, 254(1), 41-48 (1998-01-31)
A fluorometric assay of lectin binding to yeast cells is reported. The relative amount of biotinylated lectins bound to the yeast cells was estimated by enzyme activity using 4-methylumbelliferyl-beta-D-galactoside as a substrate for the lectin-bound beta-galactosidase through biotin-avidin interaction. Binding
Inter- and intra-laboratory testing of the Daphnia magna IQ toxicity test.
K R Hayes et al.
Bulletin of environmental contamination and toxicology, 57(4), 660-666 (1996-10-01)

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