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Key Documents

M4892

SAFC

McCoy 5A培养基

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

製藥生產

同義詞:

5A basal medium

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.71

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

McCoy 5A培养基推荐用于在原代细胞培养中生产病毒。

應用

McCoy 5A培养基已用于培养:
  • 人结肠癌细胞系(HCT116、RKO、FET、CBS、HCT116b和TENN),以研究富亮氨酸重复G蛋白偶联受体(LGR5)在结肠癌转移中的作用
  • 人结肠HCT116细胞,以研究miR-224在肿瘤中的过表达
  • 成骨细胞,以研究缺钙羟基磷灰石在标准细胞培养基中的离子反应性

生化/生理作用

McCoys 5A培养基起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。

數量

按照每升培养基11.9克粉末配制。

重構

补充2.2 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


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