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Key Documents

M2520

Sigma-Aldrich

培养基 199

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

同義詞:

M199 Medium, TCM 199

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

應用

培养基199已用于:

  • 在成熟前保持卵丘-卵母细胞复合体(COC),并培养这些卵母细胞在体外成熟
  • 在德国荷斯坦奶牛卵子采集过程中清洗抽吸管
  • 培养大鼠心脏内皮细胞

數量

配制成每升培养基含 14.7 克粉末。

重構

补充 2.2 g/L 碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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