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重要文件

M0393

Sigma-Aldrich

培养基 199

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

同義詞:

M199 Medium, TCM 199

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

培养基 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

形狀

powder

技術

cell culture | mammalian: suitable

成分

HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

應用

培养基199已用于:
  • 测试和确定传感器在复杂环境中的pH灵敏度
  • 作为卵母细胞采集的培养基
  • 作为杜氏利什曼虫(LdBOB)原体培养基的组分,用于培养杜氏利什曼虫
  • 内脏脂肪组织外植体

數量

按照每升培养基10.6克粉末配制。

重構

补充0.35 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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