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M1028

Sigma-Aldrich

氯化镁 溶液

for molecular biology, 1.00 M±0.01 M

同義詞:

MgCl2, dichloromagnesium, magnesium chloride

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About This Item

線性公式:
MgCl2
CAS號碼:
7786-30-3
分子量：:
95.21
MDL號碼:
MFCD00011106
分類程式碼代碼:
12352302
PubChem物質ID:
24896582
NACRES:
NA.52

等級

Molecular Biology
for molecular biology

品質等級

300

無菌

non-sterile; 0.2 μm filtered

形狀

liquid

濃度

1.00 M±0.01 M

技術

DNA sequencing: suitable

雜質

DNAse, none detected
 NICKase, none detected
 Protease, none detected
 RNAse, none detected

溶解度

water: soluble

密度

1.080 g/cm3

正離子痕跡

heavy metals (as Pb): ≤5 ppm

適合性

suitable for electrophoresis
suitable for molecular biology

應用

cell analysis

異物活動

DNase, RNase, NICKase and protease, none detected

SMILES 字串

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI 密鑰

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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相關類別

一般說明

氯化镁是一种常见的离子型卤化物，易溶于水。它在酶学、细胞膜/细胞壁结构、肌肉细胞生理学和核酸结构中起多种重要的生物学作用。此外，氯化镁在许多酶中都是基本的辅酶，包括脱氧核糖核酸酶、某些限制性酶和核糖核酸酶H。氯化镁在各种分子生物学应用中（包括PCR反应）广泛用于提供镁离子。

應用

氯化镁在各种分子生物学应用，包括PCR反应和缓冲液中提供镁离子。用途：
  • 配制免疫沉淀实验的细胞裂解缓冲液
  • 加入Krebs-Henseleit缓冲液，用于老年小鼠的长期高脂饮食研究。
  • 用于三维微生物转谷氨酰胺酶水凝胶中的细胞透化，进行骨细胞分化及互连细胞网络的体外研究。
  • 保护肝脏免遭氧化损伤。
  • 制备无血清培养基，用于大鼠脑切片培养物的电生理研究。

特點和優勢

  • 即用型溶液，免去配制步骤，节约时间。
  • 重金属（以铅计）：≤5 ppm
  • 不含DNA酶、RNA酶、切口酶和蛋白酶

成分

氯化镁溶液是含有1M氯化镁的18兆欧水。

其他說明

如需了解生化试剂系列的更多信息，请填写此表。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

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分析證明 (COA)

Lot/Batch Number
SLCL4409
WXBD6598V
SLCL2579
SLCK1344
SLCK0066

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Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

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