推薦產品
生物源
Bandieraea simplicifolia
品質等級
共軛
peroxidase conjugate
化驗
≥90% protein basis (Warburg-Christian)
形狀
lyophilized powder
效力
<200 μg per mL agglutination activity (using human blood group B erythrocytes)
peroxidase activity
40-160 units/mg protein
成份
Protein, ~95% modified Warburg-Christian
技術
blood typing: suitable
fractionation: suitable
儲存溫度
−20°C
尋找類似的產品？ 前往 產品比較指南
相關類別
應用
通用蛋白质印迹方案：
- 糖蛋白样品规格：500ng
- 凝集素浓度： 0.1ug/ml
- 每条泳道上样500 ng糖蛋白样品
- 进行4-20% Bis-Tris SDS page凝胶
- 转膜凝胶至PVDF膜上
- 用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时
- 在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时
- 用25ml RIPA缓冲液洗膜 5 x 5分钟
- 用化学发光底物（CPS1-120）进行检测
生化／生理作用
BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
其他說明
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A4 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
單位定義
在20℃、pH值为6.0条件下，1个单位的连苯三酚在20 s内会形成1 mg红棓酚。
外觀
含柠檬酸钠缓冲盐和氯化钙
準備報告
使用O′Sullivan等人的方法的改进，由VI型过氧化物酶制备。偶联后通过亲和层析重新纯化。
分析報告
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水（pH6.8）对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
客戶也查看了
Dariusz Orlowski et al.
Neuromodulation : journal of the International Neuromodulation Society, 20(5), 417-423 (2017-02-22)
The use of Deep Brain Stimulation (DBS) in treatment of various brain disorders is constantly growing; however, the number of studies of the reaction of the brain tissue toward implanted leads is still limited. Therefore, the aim of our study
Luís Fernándes de Castro et al.
Tissue engineering. Part A, 16(4), 1157-1168 (2009-10-29)
The parathyroid-hormone-related protein (PTHrP) is an important modulator of bone formation and bone remodeling. High and/or prolonged glucocorticoid (GC) treatments inhibit PTHrP expression in osteoblastic cells and bone formation and repair. We assessed the ability of the N- and C-terminal
Qing Wang et al.
Neurobiology of disease, 36(1), 223-231 (2009-07-29)
Pyruvate, a key intermediate in glucose metabolism, was explored as a potential treatment in models of experimental stroke and inflammation. Pyruvate was administered to rodents after the onset of middle cerebral artery occlusion (MCAO). Since the extent of inflammation is
Inflammation and NFkappaB activation is decreased by hypothermia following global cerebral ischemia.
Carla M Webster et al.
Neurobiology of disease, 33(2), 301-312 (2008-12-10)
We previously showed that hypothermia attenuates inflammation in focal cerebral ischemia (FCI) by suppressing activating kinases of nuclear factor-kappa B (NFkappaB). Here we characterize the inflammatory response in global cerebral ischemia (GCI), and the influence of mild hypothermia. Rodents were
Bruno Sevá Pessôa et al.
Journal of the American Heart Association, 4(2) (2015-02-07)
Angiotensin-(1-7) improves cardiac function and remodeling after myocardial infarction (MI). This may involve recruitment of hematopoietic progenitor cells that support angiogenesis. However, angiotensin-(1-7) is rapidly metabolized in plasma and tissue. The authors investigated in mice the effect of a metabolically
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務