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Key Documents

L5024

Sigma-Aldrich

脂多糖 来源于大肠杆菌 0127:B8

purified by ion-exchange chromatography, TLR ligand tested

同義詞:

LPS

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

生物源

Escherichia coli (O127:B8)

品質等級

形狀

lyophilized powder

純化經由

ion-exchange chromatography

雜質

<1% Protein
<1% RNA

顏色

white to yellow cast

溶解度

water: soluble

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

该产物从大肠杆菌血清型 O127:B8 中提取,并通过离子交换纯化。源菌株为 ATCC 12740。该 LPS 血清型已经用于脓毒性休克的研究,并且可在大鼠肾小球 masangial 细胞中诱导小鼠巨噬细胞和 PAF 中的 NOS 合成。

應用

脂多糖(LPS)是革兰氏阴性菌细胞壁的特征组分。LPS及其脂质A部分可通过能够识别常见的病原体相关分子模式(PAMP)的Toll样受体蛋白家族成员Toll样受体4(TLR4)刺激先天性免疫系统细胞。

生化/生理作用

脂多糖(LPS)位于膜的外层,并且在非包封的菌株中暴露在细胞表面上。它们有助于外膜的完整性,并保护细胞免受胆汁盐和亲脂性抗生素的作用。

準備報告

产物可溶于水(5 mg/mL)或细胞培养基(1 mg/mL),产生浑浊的淡黄色溶液。在经过涡旋,并升温至 70-80°C 后,含水盐水变得更浓缩,但仍然浑浊(20mg/mL)。脂多糖分子在每种溶剂中均形成胶束。在水和磷酸盐缓冲盐水中观察到浑浊的溶液。使用有机溶剂,溶液也是浑浊的。甲醇产生具有漂浮物的浑浊悬浮液,而水则产生均匀浑浊的溶液。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形圖

Skull and crossbones

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 2 Oral

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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存取文件庫

Mingju Cao et al.
Frontiers in cellular neuroscience, 9, 294-294 (2015-08-25)
Neuroinflammation in utero may result in life-long neurological disabilities. The molecular mechanisms whereby microglia contribute to this response remain incompletely understood. Lipopolysaccharide (LPS) or saline were administered intravenously to non-anesthetized chronically instrumented near-term fetal sheep to model fetal inflammation in
M Cortes et al.
Scientific reports, 7(1), 10645-10645 (2017-09-08)
Neuroinflammation in utero may result in life-long neurological disabilities. Microglia play a pivotal role, but the mechanisms are poorly understood. No early postnatal treatment strategies exist to enhance neuroprotective potential of microglia. We hypothesized that agonism on α7 nicotinic acetylcholine
Kristen R Hollinger et al.
Journal of Alzheimer's disease : JAD, 77(1), 437-447 (2020-07-18)
Given the emergent aging population, the identification of effective treatments for Alzheimer's disease (AD) is critical. We investigated the therapeutic efficacy of JHU-083, a brain-penetrable glutamine antagonist, in treating AD using the humanized APOE4 knock-in mouse model. Cell culture studies
Manman Geng et al.
Clinical immunology (Orlando, Fla.), 220, 108579-108579 (2020-09-01)
Endoplasmic reticulum (ER) stress associated proteins contribute to the pathogenesis of rheumatoid arthritis (RA) through affecting synoviocyte proliferation and proinflammatory cytokine production. The role of DERL3, an ER-associated degradation component, in joint inflammation of RA was explored. Synovial tissues from
Chen Seng Ng et al.
Cell death and differentiation, 27(8), 2363-2382 (2020-02-09)
Host nucleases are implicated in antiviral response through the processing of pathogen-derived nucleic acids. Among many host RNases, decapping enzymes DCP1 and 2, and 5'→3' exonuclease XRN1, which are components of the RNA decay machinery, have been extensively studied in

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