L4669

Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-LOX, Anti-Protein lysine 6-oxidase
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~50 kDa
• 物種活性: human
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1 mg/mL
• 技術: western blot: 1.5-3.0 mg/mL using HEK-293T cellls expressing human LOX
• UniProt登錄號: P28300
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LOX(4015) ; mouse ... Lox(16948) ; rat ... Lox(24914)

描述

一般說明

The LOX gene is located on the human chromosome at 5q23.1.

The gene LOX (lysyl oxidase) encodes a copper-dependent amine oxidase that belongs to LOX family of proteins. These proteins contain highly conserved C- terminal mature catalytic domains that include the copper binding site, the lysyl tyrosyl quinine (LTQ) cofactor residues, and the cytokine receptor like (CRL) domain. It is present in both intercellular and intracellular locations.

特異性

Anti-Lysyl Oxidase (C-terminal) specifically recognizes human lysyl oxidase (LOX).

應用

Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence staining
• immunohistochemistry

生化／生理作用

Altered LOX gene expression and activity are linked to skin aging and senescence. Decreased LOX gene expression and activity have been associated with severe connective tissue disorders such as Ehler-Danlos syndrome, cutis laxa, and Menkes′ syndrome. Increased LOX gene expression are associated with the development of fibrotic diseases that involve connective tissue remodeling, such as atherosclerosis, scleroderma and liver cirrhosis. LOX protein also plays an important role in breast cancer metastasis.

LOX is implicated in various intracellular functions such as the regulation of cellular differentiation, cell migration and gene transcription.

The gene LOX (lysyl oxidase) encodes an extracellular matrix remodeling enzyme that catalyzes the oxidation of primary amino group of peptidyl lysine to reactive peptidyl aldehydes. It is mainly involved in the oxidation of lysine residues in elastin and collagen, resulting in the formation of covalent cross-linkages, which stabilize these fibrous proteins. It also plays a role in several cellular processes such as developmental regulation, tumor suppression, cell motility, and cellular senescence. It is involved in hypoxia-induced metastasis and serves as a therapeutic target for the treatment of metastases.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)