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Key Documents

L4417

Sigma-Aldrich

Anti-phospho-Lck (pTyr192) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.44

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

human

技術

western blot: 1:1,000 using full length recombinant human Lck protein.

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

phosphorylation (pTyr192)

基因資訊

human ... LCK(3932)
mouse ... Lck(16818)

一般說明

Lck, also referred to as T cell-specific protein tyrosine kinase, is a 56kD protein that belongs to Src family of non-receptor tyrosine protein kinases and is expressed mainly in T cells. It has a pivotal role in the initiation of pre-TCR and T-cell antigen receptor (TCR)-linked signal transduction pathways. Anti-phospho-Lck (pTyr192) antibody reacts specifically with Lck (pTyr192) in humans.

免疫原

synthetic phosphopeptide derived from the region of Lck that is phosphorylated on tyrosine 192. Human and mouse have 100% homology.

應用

Anti-phospho-Lck (pTyr192) antibody can be used in western blotting (diluted 1:1000) using full length recombinant human Lck protein.

外觀

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide.

準備報告

The antibody is preadsorbed to remove any reactivity towards a non-phosphorylated Lck.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Hong Wang et al.
Cellular signalling, 23(1), 249-258 (2010-09-21)
Pre-T-cell receptor (pre-TCR) and TCR signals govern the development of T-lymphocytes. RhoH, a hematopoietic-specific and GTPase-deficient member of the RhoGTPase family, is required in the development of T-lymphocytes. Here we found that RhoH binds and modulates LCK, the non-receptor tyrosine

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