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Key Documents

L1394

Sigma-Aldrich

来自 普通小麦 的凝集素(小麦)

agarose conjugate, saline suspension

同義詞:

WGA, Wheat germ agglutinin

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

共軛

agarose conjugate

品質等級

形狀

saline suspension

標籤範圍

~6 mg per mL

基質

4% agarose

基質活化

cyanogen bromide

基質墊片

1 atom

粒徑

45-165 μm

容量

≥0.5 mg/mL binding capacity (ovomucoid)

儲存溫度

2-8°C

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相關類別

應用

用于纯化含有(D-N-乙酰氨基葡萄糖)2或N-乙酰神经氨酸的糖蛋白。

生化/生理作用

WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。

外觀

含15 mM叠氮化钠的0.9% NaCl中的混悬剂。

準備報告

由亲和纯化凝集素制备偶联物。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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Bo F Hansen et al.
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Liheng Yang et al.
The Journal of biological chemistry, 291(8), 3905-3917 (2015-12-20)
The normal cellular prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein. However, in pancreatic ductal adenocarcinoma cell lines, such as BxPC-3, PrP exists as a pro-PrP retaining its glycosylphosphatidylinositol (GPI) peptide signaling sequence. Here, we report the identification
D L Christie et al.
The Biochemical journal, 270(1), 57-61 (1990-08-15)
Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
María-Ángeles Cortés-Gómez et al.
International journal of molecular sciences, 24(2) (2023-01-22)
In Alzheimer's disease (AD), the reduction in acetylcholinesterase (AChE) enzymatic activity is not paralleled with changes in its protein levels, suggesting the presence of a considerable enzymatically inactive pool in the brain. In the present study, we validated previous findings
Cynthia Tang et al.
Communications biology, 4(1), 161-161 (2021-02-07)
Mutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, a lysosomal storage disorder characterized by childhood-onset vision loss, neurological impairment, and premature death. However, how CLN3 mutations cause photoreceptor cell death is not known. Here, we show that

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This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin from GE Healthcare.

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