I3769

Insulin-like Growth Factor-I human

≥95% (HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 同義詞: somatomedin c
• MDL號碼: MFCD00081716
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 產品名稱: Insulin-like Growth Factor-I human, IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 化驗: ≥95% (HPLC)
• 形狀: lyophilized powder
• 效力: 0.1-10 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: ~7.6 kDa
• 包裝: pkg of 50 and 100 μg
• 技術: cell culture | mammalian: suitable
• 雜質: ≤1.00 EU/μg
• UniProt登錄號: P05019
• 儲存溫度: −20°C
• 基因資訊: human ... IGF1(3479) , Igf1(16000)

描述

一般說明

Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.

應用

Insulin-like Growth Factor-I human has been used:

• in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
• in culture media, to study its effect on steroidogenesis during testicular development
• in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation

生化／生理作用

IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.

IGF-I, also known as somatomedin C, mediates the growth-promoting activity of GH (growth hormone). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. IGF-I exerts its actions through the IGF-I receptor.

Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.

外觀

Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.

重構

Reconstitute in water

分析報告

The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED₅₀ corresponds to a specific activity of at least 1 x 10⁵ units/mg.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)