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I3146

Sigma-Aldrich

ITS液体培养基补充剂（100x）

liquid, sterile-filtered, BioReagent, suitable for cell culture

同義詞:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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About This Item

分類程式碼代碼:
12352205
NACRES:
NA.75

無菌

sterile-filtered

品質等級

500

產品線

BioReagent

形狀

liquid

技術

cell culture | mammalian: suitable
single cell analysis: suitable

雜質

endotoxin, tested

運輸包裝

ambient

儲存溫度

2-8°C

相關類別

應用

胰岛素-转铁蛋白-硒添加剂（100×ITS液体培养基添加剂）作为培养基补充剂适用于：
  • α小鼠肝12细胞(AML-12)
  • 大鼠胎儿睾丸外植体
  • 人牙髓干细胞(DPSC)

生化／生理作用

  • 胰岛素：一种多肽激素，可促进葡萄糖和氨基酸的摄取，这种特性可能与观察到的有丝分裂作用有关。
  • 转铁蛋白：铁运输蛋白。
  • 硒：通常由血清提供的必需微量元素。

成分

含有1.0 mg/ml人重组胰岛素，0.55 mg/ml 人转铁蛋白（基本上不含铁）以及0.5 μg/ml 100x浓度的亚硒酸钠。

其他說明

ITS是人重组胰岛素、人转铁蛋白和亚硒酸钠的一种混合物。它是一种通用的细胞补充剂设计用于含有丙酮酸钠的非复合型培养基（如MEM、RPMI-1640）及复合型培养基（如Ham?s F-12、DME/F-12、MEM）。

準備報告

使用不含酚红的Earle′平衡盐溶液（EBSS）制备。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Kelvin S C Cheung et al.
PloS one, 9(6), e98063-e98063 (2014-06-04)
MicroRNAs (miRs) play a pivotal role in a variety of biological processes including stem cell differentiation and function. Human foetal femur derived skeletal stem cells (SSCs) display enhanced proliferation and multipotential capacity indicating excellent potential as candidates for tissue engineering
Leonardo Ricotti et al.
Biomedical materials (Bristol, England), 6(3), 031001-031001 (2011-05-14)
Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro
Christine M Reich et al.
Veterinary research communications, 36(2), 139-148 (2012-03-07)
In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrow-derived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent
Zhibin Lin et al.
Cell death & disease, 11(1), 58-58 (2020-01-25)
Translationally controlled tumor protein (TCTP), which is a protein characterized by its potent proliferation promoting activity, has been well studied in the area of growth and tumorigenesis. However, the specific role of TCTP in liver regeneration (LR) and its underlying
Yi Yu et al.
Scientific reports, 7(1), 1729-1729 (2017-05-13)
Hepatitis B virus (HBV) infection causes acute hepatitis B (AHB), chronic hepatitis B (CHB), liver cirrhosis (LC), and eventually hepatocellular carcinoma (HCC). The presence of hepatitis B e antigen (HBeAg) in the serum generally indicates ongoing viral replication and disease
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细胞培养中的转铁蛋白

转铁蛋白和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统（用于生物制造包括单克隆抗体在内的异源蛋白）的性能。本页介绍转铁蛋白的体外化学和生物化学。

Transferrin in Cell Culture

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