I2276

Interleukin-12 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• 同義詞: IL-12
• MDL號碼: MFCD00212975
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 化驗: ≥97% (SDS-PAGE and N-terminal analysis)
• 形狀: lyophilized powder
• 效力: 0.01-0.05 ng/mL ED₅₀
• 分子量: glycoprotein 75 kDa
• 包裝: pkg of 5 μg
• 技術: cell culture | mammalian: suitable
• 雜質: endotoxin, tested
• UniProt登錄號: P29459,; P29460
• 儲存溫度: −20°C
• 基因資訊: human ... IL12A(3592) , IL12B(3593)

描述

生化／生理作用

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

外觀

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)