推薦產品
一般說明
提供1步法和2步法RT-PCR反应。
1步法： 同一管中的eAMV™ RT逆转录酶和AccuTaq LA DNA聚合酶相继发挥作用，首先生成cDNA，紧接着进行PCR扩增反应。 用于灵敏、快速的RNA分析。
2步法： 每次反应都经过单独优化，可在高保真前提下获得更高产率。适合需要更多扩增的实验，或高产率比便利性更加重要的实验。
1步法： 同一管中的eAMV™ RT逆转录酶和AccuTaq LA DNA聚合酶相继发挥作用，首先生成cDNA，紧接着进行PCR扩增反应。 用于灵敏、快速的RNA分析。
2步法： 每次反应都经过单独优化，可在高保真前提下获得更高产率。适合需要更多扩增的实验，或高产率比便利性更加重要的实验。
應用
增强型禽类HS RT-PCR试剂盒用于合成反转录酶PCR (RT-PCR) 分析的第一条cDNA链。
特點和優勢
- 该反转录酶可制备更长的转录本，生成长达14.1kb的cDNA。
- 低丰度mRNA检测灵敏度更高。eAMV™ RT能够成功转录其他反转录酶无法检测到的微量RNA。
- 针对复杂的二级结构的RNA,拥有超强的转录能力。
- JumpStart AccuTaq LA DNA聚合酶有利于提高灵敏度、特异性与得率。
其他說明
Reverse Transcriptase PCR (RT-PCR)是一种强大的基因表达研究工具。Enhanced Avian HS RT-PCR试剂盒应用了一款加强型禽成髓细胞瘤病毒逆转录酶（eAMV-RT），相比标准型AMV-RT和MMLV-RT具有更佳的反应性能。eAMV RT是一种效率极强的酶，在较高温度下（65 °C）仍能够打开复杂RNA的二级结构成功介导转录反应，是以总RNA或poly（A）+ RNA为模板制备高质量全长cDNA（长达14.1kb）的最佳酶蛋白。同时本品也附赠JumpStartTM ™AccuTaq™ LA DNA聚合酶混合物，可减少非特异性扩增，增加反应特异性与灵敏度。这两种酶的组合为用户提供了一个高品质的反应系统，能够同时兼容一步法或两步法的RT-PCR方案。
法律資訊
No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
eAMV is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
The secreted integrin ligand nephronectin is necessary for forelimb formation in Xenopus tropicalis.
Anita Abu-Daya et al.
Developmental biology, 349(2), 204-212 (2010-10-28)
While limb regeneration has been extensively studied in amphibians, little is known about the initial events in limb formation in metamorphosing anurans. The small secreted integrin ligand nephronectin (npnt) is necessary for development of the metanephros in mouse. Although expressed
17-estradiol attenuates LPS-induced interleukin-8 production by human peripheral blood monocytes through estrogen receptor-activation
Malisorn N, et al.
African Journal of Pharmacy and Pharmacology, 4(11), 806-810 (2010)
Samantha Reese et al.
Journal of fungi (Basel, Switzerland), 7(7) (2021-07-03)
Fungal cell wall receptors relay messages about the state of the cell wall to the nucleus through the Cell Wall Integrity Signaling (CWIS) pathway. The ultimate role of the CWIS pathway is to coordinate repair of cell wall damage and
Nicotiana tabacum osmotic stress-activated kinase is regulated by phosphorylation on Ser-154 and Ser-158 in the kinase activation loop
Burza AM, et al.
The Journal of Biological Chemistry, 281(45), 34299-34311 (2006)
Chelsea T Smartt et al.
The American journal of tropical medicine and hygiene, 81(2), 258-263 (2009-07-29)
Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding
條款
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務