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Key Documents

H9286

Sigma-Aldrich

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

同義詞:

Anti-H3K9ac

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen ~17 kDa

物種活性

bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken

濃度

2-2.5 mg/mL

技術

indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
microarray: suitable
western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

acetylation (Lys9)

一般說明

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:
  • indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
  • microarray
  • western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

免疫原

synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

應用

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:
  • indirect immunofluorescence
  • immunoblotting
  • immunohistochemistry

生化/生理作用

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.
In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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