H6660

HIV Protease Substrate 1

≥95% (HPLC), powder

• 同義詞: Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg
• MDL號碼: MFCD00283442
• 分類程式碼代碼: 12352204
• NACRES: NA.32

屬性

• 產品名稱: HIV Protease Substrate 1,
• 化驗: ≥95% (HPLC)
• 品質等級: 200
• 形狀: powder
• 分子量: 2016
• 溶解度: DMSO: soluble
• 儲存溫度: −20°C

描述

應用

HIV Protease Substrate 1 has been used:

• to study protein structure-function relationships and in the fluorescent assay
• to evaluate protease inhibitors and for in vitro detection of the HIV-1 protease activity
• and in protease kinetics to study its enzymatic degradation rates

生化／生理作用

HIV Protease Substrate 1 is a synthetic peptide sequence that contains the cleavage site (Tyr-Pro) for the human immunodeficiency virus (HIV) Protease. It has two covalently modified amino acids for the detection of cleavage. One modification is the addition of the fluorophore 5-(2-aminoethylamino)-1-naphthalene sulfonate (EDANS) to the glutamic acid residue, while the other one includes the addition of the acceptor chromophore 4c-dimethylaminoazobenzene-4-carboxylate (DABCYL) to the lysine residue. The modified amino acids are on opposite sides of the cleavage site. Spatial orientation and overlap of the DABCYL absorbance with the EDANS emission permit resonance energy transfer between the two moieties. However, when the peptide is cleaved by the HIV protease, the DABCYL group is no longer proximal to the fluorophore.

Substrate for endopeptidases

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)