推薦產品
生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 55 kDa
物種活性
mouse, human
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections
immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells
microarray: suitable
western blot: 1:20,000 using nuclear extract from human HeLa cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... HDAC2(3066)
mouse ... Hdac2(15182)
一般說明
Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.
特異性
Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.
免疫原
synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).
應用
Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:
- in immunoblotting and dot blot
- in immunoprecipitation
- in immunohistochemistry
- in enzyme linked immunosorbent assay (ELISA)
- in western blotting
- in chromatin immunoprecipitation(ChiP)
生化／生理作用
Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
外觀
Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NF-kappaB suppression
Ito K, et al.
The Journal of Experimental Medicine, 203(1), 7-13 (2006)
Anticancer activities of histone deacetylase inhibitors
Bolden J E, et al.
Nature Reviews Drug Discovery, 5(9), 769-769 (2006)
Development of an ELISA-based HDAC activity assay for characterization of isoform-selective inhibitors
Padige G, et al.
Journal of Biomolecular Screening, 20(10), 1277-1285 (2015)
DNA methyltransferase 3b preferentially associates with condensed chromatin
Kashiwagi K, et al.
Nucleic Acids Research, 39(3), 874-888 (2010)
SAHA decreases HDAC 2 and 4 levels in vivo and improves molecular phenotypes in the R6/2 mouse model of Huntington's disease
Mielcarek M, et al.
PLoS ONE, 6(11), e27746-e27746 (2011)
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