推薦產品
描述
Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White
特點
wetted part: no
包裝
pkg of 1 kit
製造商／商標名
Cytiva RPN2108
儲存溫度
2-8°C
一般說明
ECL™ Western Blotting System is the world′s most widely used and referenced chemiluminescent immunodetection system.
ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.
ECL™ Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL™ Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL™ Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.
應用
ECL™ Blotting Analysis System has been used in western blotting.
Western Blotting
特點和優勢
- Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL™ reagents.
- Optimized system provides rapid, sensitive results using simple protocols.
- Designed for use with nitrocellulose and PVDF membranes.
- High sensitivity: Up to 10 times more sensitive than colorimetric methods.
- Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
- Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
- Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
- Non-radioactive-no specialized facilities needed
- Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
ECL is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
12 - Non Combustible Liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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